Ikhithi Yokuhlola I-Novel Coronavirus (2019-nCoV) ye-Nucleic Acid
Imininingwane Yomkhiqizo:
I-Innovita® 2019-nCoV IgM/IgG Test ihloselwe ukuxilonga nokuqapha izifo ezibangelwa i-coronavirus yenoveli (2019-nCoV).I-ORF1ab kanye ne-N gene ye-2019-nCoV zitholwa ngokufanelekile kuma-swabs omphimbo kanye namasampula oketshezi lwe-alveolar lavage aqoqwe ezimweni okusolwa ukuthi zenyumoniya, iziguli ezinamacala asolwayo, abanye abadinga ukuxilonga.
Imiphumela yokuhlolwa yale khithi ingeyenkomba yomtholampilo kuphela.Kunconywa ukwenza ukuhlaziywa okuphelele kwesimo ngokusekelwe ekubonakalisweni komtholampilo kwesiguli kanye nezinye izivivinyo zaselabhorethri.
Isimiso:
Le kit isebenzisa isinyathelo esisodwa sokuhlehla sokuloba i-polymerase chain reaction (RT-I-PCR) ubuchwepheshe bokuthola ukuze kuqondiswe inoveli coronavirus (2019-nCoV) I-ORF1ab gene, i-N gene, kanye nokulandelana kofuzo kwangaphakathi komuntu okuyisethenjwa.Ama-primers athile kanye nama-taqman probe aklanywe ezifundeni ezilondoloziwe.
Ukwakhiwa:
Ukwakheka | 48 ukuhlolwa / ikhithi |
I-Reaction Mix A | 792μL×1Tube |
I-Reaction Mix B | 168μL×1Tube |
Ukulawula Okuhle | 50μL×1Tube |
Ukulawula Okungalungile | 50μL ×1Tube |
Qaphela: 1. Amaqoqo ahlukene ama-reagents akufanele ahlanganiswe.
2. Ukulawula okuhle nokulawula okuhle akudingekile ukuthi kukhishwe
Inqubo Yokuhlola:
1. Ukukhishwa kwe-Nucleic acid:
Amakhithi okukhishwa kwe-RNA ezohwebo ayatholakala, ukukhipha ubuhlalu obuzibuthe kanye nokukhipha ikholomu e-spin kuyanconywa kule khithi.
2. Lungiselela Imiksi Yokusabela:
● Khipha i-2019-nCoV reaction Mix A/B futhi ugcine kuzinga lokushisa elilingana negumbi kuze kube yilapho kuqandisiwe;
● Thatha izingxenye ezihambisanayo (I-Reaction Mix A 16.5μL/T, I-Reaction Mix B 3.5μL/T) bese uxuba, bese uliquot ukusabela ngakunye kwe-PCR no-20μL/ishubhu;
● Engeza u-5μL wesifanekiso se-RNA noma isilawuli esinegethivu noma isilawuli esihle, bese uvala isivalo seshubhu;
● Beka ishubhu yokusabela ensimbini ye-fluorescence PCR, bese usetha isilawuli esinegethivu / esihle kanye nemingcele yesampula yokusabela kwe-RT-PCR ngokuya ngeziyalezo zokusebenza kwethuluzi.
● Rekhoda i-oda lokubekwa kwesampula
3.RT-PCR amaphrothokholi:
Izilungiselelo Ezinconyiwe:
Umjikelezo | Isikhathi | Izinga lokushisa(℃) | |
1 | 1 | 10 imiz | 25 |
2 | 1 | 10 imiz | 50 |
3 | 1 | 10 imiz | 95 |
4 | 45 | 10s | 95 |
35s | 55 |