Ukuhlolwa kwe-2019-nCoV Neutralizing Antibody (QDIC)
Imininingwane Yomkhiqizo:
Ukuhlolwa kwe-Innovita® 2019-nCoV IgM/IgG kuhloselwe ukutholwa kwenani lokungavikeleki kwamasosha omzimba ku-noveli coronavirus (2019-nCoV) ku-serum yomuntu, i-plasma noma igazi eliphelele (igazi lomunwe noma igazi eligcwele le-venous).
I-2019-nCoV ifaka amaprotheni esakhiwo amane abalulekile: amaprotheni e-S, i-E protein, i-M protein kanye ne-N protein.Isifunda se-RBD se-S protein singabophezela ku-cell surface receptor ACE2.Ucwaningo selukhombisile ukuthi izibonelo zabantu abeluleme ekuthelelekeni kwe-coronavirus yenoveli banethemba lokwehlisela amandla amasosha omzimba.Ukutholwa kwe-antibody engathathi hlangothi kungasetshenziswa ukuhlola ukubikezelwa kokutheleleka ngegciwane kanye nokuhlolwa komphumela ngemva kokugoma.
Isimiso:
Ikhithi iyi-quantum dot immunofluorescence chromatography assay yokuthola amasosha omzimba angama-2019-nCoV RBD akhethekile e-IgG ku-serum yomuntu, i-plasma noma igazi eliphelele (igazi lomunwe kanye negazi eligcwele le-venous).Ngemva kokuba isifanekiso sisetshenziswe esibonelweni kahle, uma ukuhlangana kwamasosha omzimba angathathi hlangothi kuphezulu kunomkhawulo wokutholwa ophansi kakhulu, amasosha omzimba athile e-RBD e-IgG azosabela ngengxenye noma yonke i-antigen ye-RBD ebhalwe nge-quantum dot microspheres ukuze akhe isakhiwo sokuzivikela ezifweni.Khona-ke i-immune compound izofuduka eduze kwe-nitrocellulose membrane.Lapho befika endaweni yokuhlola (umugqa we-T), inhlanganisela izosabela ngeketango le-anti-human IgG (γ) elimbozwe kulwelwesi lwe-nitrocellulose futhi yakhe umugqa we-fluorescent.Funda inani lesignali ye-fluorescence nge-fluorescence immunoassay analyzer.Inani lesignali lilingana nokuqukethwe kwama-antibody anciphisa kusifanekiso.
Ukuthi isampula iqukethe amasosha omzimba akhethekile e-RBD noma cha, ulayini wokulawula kufanele uhlale uvela ewindini lomphumela uma inqubo yokuhlola yenziwe kahle futhi i-reagent isebenza ngendlela ehlosiwe.Lapho i-antibody yenkukhu ye-IgY ebhalwe nge-quantum dot microspheres ithuthela kulayini wokulawula (C line), izobanjwa yi-anti-chicken IgY antibody efakwe ngaphambili kulayini C, bese kwakhiwa umugqa we-fluorescent.Ulayini wokulawula (umugqa C) usetshenziswa njengokulawula inqubo.
Ukwakhiwa:
| Ukwakheka | Inani | Ukucaciswa |
| IFU | 1 | / |
| Hlola ikhasethi | 20 | Isikhwama ngasinye se-foil esivaliwe esiqukethe idivayisi eyodwa yokuhlola kanye ne-desiccant eyodwa |
| I-dilumen yesampula | 3mL*1 ibhodlela | 20mM PBS, Sodium Casein, ProClin 300 |
| I-Micropipette | 20 | I-Micropipette enomugqa womaka ongu-20μL |
| I-Lancet | 20 | / |
| Iphedi lotshwala | 20 | / |
Inqubo Yokuhlola:
● Iqoqo Legazi Lomunwe
● Funda umphumela nge-analyzer ye-fluorescence
