Imikhiqizo

Imininingwane Yomkhiqizo:

I-Innovita® 2019-nCoV IgM/IgG Test ihloselwe ukutholwa kwenani elincane lamasosha omzimba angathathi hlangothi ku-coronavirus yenoveli (2019-nCoV) ku-serum yomuntu, i-plasma noma isampula yegazi lonke.
I-2019-nCoV ifaka amaprotheni esakhiwo amane abalulekile: amaprotheni e-S, i-E protein, i-M protein kanye ne-N protein.Isifunda se-RBD se-S protein singabophezela ku-cell surface receptor ACE2.I-antibody engathathi hlangothi ibhekisela emandleni okuhlangana ne-pathogen, bese ivimbela i-pathogen ukuze ihlasele umzimba ukuze ibangele ukutheleleka.Ukutholwa kwe-antibody engathathi hlangothi kungasetshenziswa ukuhlola ukubikezelwa kokutheleleka ngegciwane.

Isimiso:

Ikhithi iyisivivinyo somncintiswano we-colloid gold immunochromatography ukuthola amasosha omzimba angathathi hlangothi ku-2019-nCoV ku-serum yomuntu, i-plasma noma isampula yegazi lonke.Ngemva kokuba isifanekiso sisetshenziswe esibonelweni kahle, uma amasosha omzimba angathathi hlangothi ekhona esibonelweni, amasosha omzimba angathathi hlangothi azosabela ngegolide elicwengekileyo elibhalwe ukuthi i-RBD antigen ukuze kwakhe i-immune complex, kanye nesizinda sokungathathi hlangothi se-antigen ye-RBD izovalwa.Bese i-immune complex kanye ne-antigen ye-RBD ebhalwe ukuthi i-RBD ngaphandle kokubophezela ekwakhiweni kwe-antibody eshintshashintshayo ifuduka eduze kolwelwesi lwe-nitrocellulose.Lapho zifika endaweni yokuhlola (umugqa we-T), i-antigen ye-RBD enelebula ngaphandle kokubophezela emasosha omzimba azosabela nge-antigen ye-ACE2 embozwe kulwelwesi lwe-nitrocellulose futhi yakhe umugqa onsomi-obomvu.Uma ukuhlangana kwamasosha omzimba angathathi hlangothi kuphezulu kunomkhawulo wokutholwa ophansi kakhulu, ulayini onsomi-obomvu ulula kunolayini wokulawula (umugqa C) noma ungekho umugqa onsomi-obomvu owenziwe, umphumela uba muhle.Uma ukuhlangana kwamasosha omzimba angathathi hlangothi kuphansi kunomkhawulo wokutholwa ophansi kakhulu noma kungekho amasosha omzimba angathathi hlangothi kusifanekiso, umugqa onsomi-obomvu uba mnyama kunolayini wokulawula, umphumela uba mubi.
Noma ngabe lesi sifanekiso siqukethe amasosha omzimba angama-2019-nCoV, lapho i-antibody yenkukhu e-colloidal ebhalwe ngegolide i-IgY ithuthela kulayini wokulawula (C line), izobanjwa i-anti-chicken IgY antibody efakwe kulayini wokulawula (C). line), kwakhiwa umugqa onsomi-obomvu.Ulayini wokulawula (umugqa C) usetshenziswa njengokulawula inqubo.Imigqa yokulawula kufanele ivele kumafasitela wemiphumela uma inqubo yokuhlola yenziwe kahle futhi ama-reagents asebenza ngendlela ehlosiwe.

Ukwakhiwa:

Inqubo Yokuhlola:

1. Vula isikhwama se-aluminium foil bese ukhipha ikhasethi lokuhlola.
2. Faka u-40μL we-serum/plasma specimen noma u-60μL wegazi eliphelele emthonjeni wesampula.
3. Faka i-40μL (amaconsi ama-2) isifanekiso sokuxubha esibonelweni kahle.
4. Yibeke ekamelweni lokushisa (15℃~30℃) imizuzu engu-15-20, bese ufunda umphumela.

Ukuhunyushwa Kwemiphumela:

1. Okuhle: Uma umbala womugqa ongu-T ulula kunalowo womugqa ongu-C noma uma ungekho umugqa ongu-T, ubonisa ukuthi kuhle ekwenzeni amasosha omzimba angasebenzi.
2. Okubi: Uma umbala womugqa T umnyama kakhulu noma ulingana nalowo womugqa ongu-C, ubonisa ukuthi unegethivu ekunciphiseni amasosha omzimba.
3. Okungavumelekile: Uma ulayini C wehluleka ukuvela, kungakhathaliseki ukuthi umugqa we-T uyabonakala noma cha, ukuhlola akuvumelekile.Phinda ukuhlolwa ngohlolo olusha.