I-Rotavirus/Adenovirus/Norovirus Ag Test
Ukusetshenziswa Okuhlosiwe
Ikhithi ihloselwe ukutholwa okuqondile nokusezingeni eliphezulu kwama-antigen e-rotavirus eqembu A, ama-antigen e-adenovirus 40 no-41, ama-norovirus (GI) kanye nama-norovirus (GII) ama-antigens ezibonelweni zendle yabantu.
Umphumela wokuhlolwa othi unayo idinga ukuqinisekiswa okwengeziwe.Umphumela wokuhlolwa onegethivu awukhiphi amathuba okutheleleka.
Imiphumela yokuhlolwa yale khithi ingeyenkomba yomtholampilo kuphela.Kunconywa ukwenza ukuhlaziywa okuphelele kwesimo ngokusekelwe ekubonakalisweni komtholampilo kwesiguli kanye nezinye izivivinyo zaselabhorethri.
Isifinyezo
I-Rotavirus (RV)iyigciwane elibalulekile elidala isifo sohudo esibangelwa amagciwane kanye ne-enteritis ezinsaneni nasezinganeni ezincane emhlabeni wonke.Inani eliphakeme lezigameko lisekwindla, eyaziwa nangokuthi "uhudo lwasekwindla lwezingane kanye nezingane ezincane".Izehlakalo zezifo ezibangelwa amagciwane ezinsaneni phakathi nezinyanga neminyaka engu-2 ubudala zifinyelela ku-62%, futhi isikhathi sokufukamela siyizinsuku ezi-1 kuya kweziyi-7, ngokuvamile ngaphansi kwamahora angu-48, okubonakaliswa uhudo olunzima nokuphelelwa amandla kwamanzi.Ngemva kokuhlasela umzimba womuntu, iphindaphinda emangqamuzaneni amabi e-epithelial ethunjini futhi ikhishwe ngobuningi ngendle.
I-Adenovirus (ADV)iyigciwane le-DNA elinemicu ephindwe kabili enobubanzi obungu-70-90nm.Igciwane le-icosahedral elilinganayo elingenayo imvilophu.Izinhlayiya zegciwane ikakhulukazi zakhiwe amagobolondo amaprotheni kanye ne-DNA enemicu ephindwe kabili.I-Enteric adenovirus uhlobo 40 kanye nohlobo 41 lweqembu elincane F amagciwane abalulekile esihudo esibangelwa amagciwane kumuntu, esihlasela kakhulu izinsana nezingane ezincane (ezingaphansi kweminyaka engu-4 ubudala).Isikhathi se-incubation singaba yizinsuku ezi-3 kuya kweziyi-10.Iphindaphinda kumaseli amathumbu futhi ikhishwe endle izinsuku eziyi-10.Ukubonakaliswa komtholampilo ubuhlungu besisu, isifo sohudo, indle enamanzi, ehambisana nomkhuhlane nokuhlanza.
I-Norovirus (NoV)ungowomndeni we-caliciviridae futhi unezinhlayiya ezingama-20-hedral ezinobubanzi obungu-27-35 nm futhi ayikho imvilophu.I-Norovirus ingenye yezifo eziyinhloko ezibangela i-non-bacterial acute gastroenteritis okwamanje.Leli gciwane litheleleka kakhulu futhi lisakazwa kakhulu ngamanzi angcolile, ukudla, ukudluliswa kokuxhumana kanye ne-aerosol eyakhiwe izinto ezingcolile.I-Norovirus iyigciwane lesibili eliyinhloko elibangela isifo sohudo esibangelwa amagciwane ezinganeni, futhi liqubuka ezindaweni eziminyene.Ama-Noroviruses ahlukaniswe kakhulu abe ama-genomes amahlanu (GI, GII, GIII, GIV kanye ne-GV), kanti amagciwane amakhulu abantu yi-GI, GII kanye ne-GIV, phakathi kwawo i-GII genome okuyizinhlobo zegciwane ezivame kakhulu emhlabeni jikelele.Izindlela zokuxilonga zasemtholampilo noma zaselabhorethri zokutheleleka nge-norovirus ikakhulukazi zihlanganisa i-electron microscopy, i-molecular biology kanye nokutholwa kwe-immunological.
Ukwakheka
Ukuqoqwa nokuphathwa kwesifanekiso
1. Qoqa isifanekiso sendle esingahleliwe endaweni ehlanzekile, neyomile.
2. Vula idivayisi yokuqoqa indle ngokuqaqa phezulu bese usebenzisa ifosholo lokuqoqa ngokungahleliwe
3. bhoboza isifanekiso sendle ezindaweni ezi-2 ~ 5 ezihlukene ukuze uqoqe cishe u-100mg wendle eqinile (okulingana ne-1/2 yephizi) noma indle engu-100μL ewuketshezi.Ungakhiphi isampula yendle ngoba lokhu kungase kuholele kumphumela wokuhlola ongavumelekile.
4. Qinisekisa ukuthi i-specimen yendle isemihubheni yefosholo lokuqoqwa.Isampula yendle eyeqile ingase iholele kumphumela wokuhlola ongavumelekile.
5. Vula futhi uqinise ikepisi kudivayisi yokuqoqa isifanekiso.
6. Shake idivayisi yokuqoqa indle ngamandla.
Inqubo Yokuhlola
1. Letha isifanekiso nezingxenye zokuhlola ekamelweni lokushisa uma siqandisiwe noma siqandisiwe.
2. Uma usulungele ukuqala ukuhlola, vula isikhwama esivaliwe ngokudabula inothi.Susa isivivinyo esikhwameni.
3. Beka idivayisi yokuhlola endaweni ehlanzekile, eyisicaba.
4. Misa idivayisi yokuqoqa indle iqonde futhi usonte isivalo se-dispenser.
5. Ubambe idivayisi yokuqoqa indle uqonde phezulu, sebenzisa u-80μL (cishe amathonsi angu-2) wesisombululo emthonjeni wesifanekiso sedivayisi yokuhlola.Ungalayishi kakhulu isifanekiso.
6. Funda umphumela wokuhlolwa phakathi nemizuzu eyi-15.Ungawufundi umphumela ngemuva kwemizuzu eyi-15.
Ukuhunyushwa Kwemiphumela
1. Okuhle:Ukuba khona kwemigqa emibili ebomvu-nsomi (T no-C) ngaphakathi kwewindi lomphumela kukhombisa ukuthi kuhle ku-antigen ye-RV/ADV/NoV.
2. Okubi:Umugqa owodwa kuphela obomvu-nsomi ovela kulayini wokulawula (C) okhombisa umphumela ongemuhle.
3. Akuvumelekile:Uma ulayini wokulawula (C) wehluleka ukuvela, kungakhathaliseki ukuthi ulayini we-T uyabonakala noma cha, ukuhlolwa akuvumelekile.Buyekeza inqubo bese uphinda ukuhlola ngedivayisi yokuhlola entsha.
